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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARAYCOCHEA, S.; SPERANZA, P.; ALVAREZ-VALIN, F. |
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Afiliación : |
SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Applications in Plant Sciences, 2015, v.3, no.10, Article number 1500022. OPEN ACCESS |
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ISSN : |
2168-0450 |
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DOI : |
10.3732/apps.1500022 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 12 October 2015.
The authors thank Fabián Capdeville for his important support at the beginning of this work. This project received financial support from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. S.G. was the recipient of a Master's degree fellowship awarded by Agencia Nacional de Investigación e Innovación (ANII), Uruguay. F.A.?V. and P.S. are researchers from Sistema Nacional de Investigadores (ANII, Uruguay). |
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Contenido : |
ABSTRACT.
Premise of the study: We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. Methods: A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. Results: From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs. This weedy rice was classified based on 23 polymorphic regions identified by comparison with reference chloroplast genomes. We detected recent and past events of genetic material transfer between the chloroplast and nuclear genomes and estimated their occurrence frequency. Discussion: We obtained a high-quality complete chloroplast genome sequence from low-coverage sequencing data. Intergenome DNA transfer appears to be more frequent than previously thought.
© 2015 Garaycochea et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
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Palabras claves : |
Bioinformatic methods; BIOINFORMÁTICA; Chloroplast genome; Next-generation; Sequencing; Weedy rice. |
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Thesagro : |
ARROZ; BIOLOGÍA; CIENCIA DE INFORMACIÓN; ORYZA SATIVA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5184/1/Garaycochea-S.-2015.-Applications-in-plant-Sciences.pdf
https://bsapubs.onlinelibrary.wiley.com/doi/pdf/10.3732/apps.1500022
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Marc : |
LEADER 02579naa a2200301 a 4500
001 1053875
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
022 $a2168-0450
024 7 $a10.3732/apps.1500022$2DOI
100 1 $aGARAYCOCHEA, S.
245 $aA strategy to recover a high-quality, complete plastid sequence from low-coverage whole-genome sequencing.$h[electronic resource]
260 $c2015
500 $aArticle history: First published: 12 October 2015. The authors thank Fabián Capdeville for his important support at the beginning of this work. This project received financial support from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. S.G. was the recipient of a Master's degree fellowship awarded by Agencia Nacional de Investigación e Innovación (ANII), Uruguay. F.A.?V. and P.S. are researchers from Sistema Nacional de Investigadores (ANII, Uruguay).
520 $aABSTRACT. Premise of the study: We developed a bioinformatic strategy to recover and assemble a chloroplast genome using data derived from low-coverage 454 GS FLX/Roche whole-genome sequencing. Methods: A comparative genomics approach was applied to obtain the complete chloroplast genome from a weedy biotype of rice from Uruguay. We also applied appropriate filters to discriminate reads representing novel DNA transfer events between the chloroplast and nuclear genomes. Results: From a set of 295,159 reads (96 Mb data), we assembled the chloroplast genome into two contigs. This weedy rice was classified based on 23 polymorphic regions identified by comparison with reference chloroplast genomes. We detected recent and past events of genetic material transfer between the chloroplast and nuclear genomes and estimated their occurrence frequency. Discussion: We obtained a high-quality complete chloroplast genome sequence from low-coverage sequencing data. Intergenome DNA transfer appears to be more frequent than previously thought. © 2015 Garaycochea et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
650 $aARROZ
650 $aBIOLOGÍA
650 $aCIENCIA DE INFORMACIÓN
650 $aORYZA SATIVA
653 $aBioinformatic methods
653 $aBIOINFORMÁTICA
653 $aChloroplast genome
653 $aNext-generation
653 $aSequencing
653 $aWeedy rice
700 1 $aSPERANZA, P.
700 1 $aALVAREZ-VALIN, F.
773 $tApplications in Plant Sciences, 2015$gv.3, no.10, Article number 1500022. OPEN ACCESS
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 59 | |
| 2. |  | GARAYCOCHEA, S.; ALTIER, N. Estudio de la diversidad genética de las comunidades microbianas asociadas a la dinámica del P en suelos de Uruguay. GGM 47 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 278| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
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| 12. | | ALTIER, N.; ABREO, E.; BEYHAUT, E.; RIVAS, F.; GARAYCOCHEA, S. Beneficial microbes for agriculture in Uruguay: strategies and successful case studies. (O19-3). [Abstract]. XIX International Plant Protection Congress, IPPC 2019. 10-14 November 2019, Hyderabad, Telangana, India. "Crop Protection to Outsmart Climate Change for Food Security & Environmental Conservation”. Organized by ICRISAT (International Crops Research Institute for the Semi-Arid Tropics). In: INTERNATIONAL PLANT PROTECTION CONGRESS, 19, 2019, Hyderabad, India. Abstract Book... Hyderabad: IPPC, 2019. p. 99.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 15. | | BERNASCHINA, Y.; GARAYCOCHEA, S.; FRESIA, P.; LEONI, C. Efecto del manejo de suelo en la salud del viñedo. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 27. Financiamiento: INIA - Proyecto INIA FR22: Respuesta fisiológica de frutales al estrés biótico y abiótico; componente 1: Respuestas fisiológicas de la vid al estrés hídrico y estrés biótico (Botrytis cinerea).| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| 17. | | MASTROIANNI, L.; SILVERA, M.; IRIARTE, W.; GARAYCOCHEA, S.; LEONI, C. P19. Caracterización de una colección nacional de Pseudomonas obtenidas de yemas de flor del peral. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 45. Financiamiento: INIA- Proyecto FR21 y Beca ANII de L. Mastroianni (POS_NAC_2022_2_173800). -- Autor correspondencia: e-mail: lucasmasdia@gmail.com| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 59 | |
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